tc zonguldak karaelmas üni̇versi̇tesi̇

EK-5
T.C.
ZONGULDAK KARAELMAS
ÜNİVERSİTESİ
BİLİMSEL ARAŞTIRMA PROJESİ
KESİN RAPOR
PROJE NO
PROJENİN ADI
2011-10-03-06
BAZI METAL KOMPLEKSLERİNİN DNA İLE
ETKİLEŞİMLERİNİN BELİRLENMESİ
Proje Ekibi
Yrd.Doç.Dr. Burak ÇOBAN
Arş.Gör. Ufuk YILDIZ
RAPOR TARİHİ
22/05/2012
ABSTRACT
In this work the synthesis of 1,10-phenanthroline based ligands bearing imidazole ring and
their corresponding transition metal complexes and DNA interactions of these complexes
were studied.
2-phenylimidazo[4,5-f][1,10]phenanthroline
(PIP)
and 2-(2-hydroxyphenyl)imidazo[4,5-
f][1,10]phenanthroline (HPIP) ligands were synthesized starting from 1,10-phenanthroline5,6-dion. (2,2′-bipyridine)2-phenylimidazo[4,5-f][1,10]phenanthroline)Platinium(II) ([Pt(bpy)
(PIP)]+2)
and
(2,2′-bipyridine)2-(2-hydroxyphenyl)imidazo[4,5-f][1,10]phenanthroline
Platinium(II) ([Pt(bpy)(HPIP)]+2) complexes were obtained from ligands. The ligands were
characterized by using FT-IR and H-NMR. Complexes were characterized by using ESI-MS
and H-NMR analysis.
DNA interactions of complexes were determined by UV titration, agarose gel electrophoresis,
fluorometer and viscosimeter methods. UV titration studies suggest that [Pt(bpy)(HPIP)]+2
interacts with DNA stronger than the other complex. Electrophoresis and viscosity studies
support the UV titration results. As expected the binding constant of [Pt(bpy)(HPIP)]+2 was
lower than [Pt(bpy)(HPIP)]+2 complex’s binding constant in only fluorometer studies because
of structure of [Pt(bpy)(HPIP)]+2.
All of the results suggest that both of the complexes intercalates in DNA base pairs.