TOMRİS ÖZBEN TOMASİ LABORATUVAR MALZEMESİ TEKNİK

TEKNİK ŞARTNAME
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DMEM, high glucose, with glutamine, 500 ml for cell culture
RPMI 1640 with L-Glutamine, 500 ml for cell culture.
Fetal Bovine Serum: Heat Inactivated, sterile-filtered,suitable for cell culture,
L-ASCORBIC ACID,Cell culture,100 g
Penicillin–Streptomycin Solution with 10,000 units penicillin and 10mg/ml streptomycin in 0.9%
NaCl, sterile-filtered,suitable for cell culture, BioReagent.
Trypsin-EDTA solution 10 ×, sterile-filtered, BioReagent, suitable for cell culture, 5.0 g porcine
trypsin and 2 g EDTA • 4Na per liter of 0.9% sodium chloride. sterile-filtered, suitable for cell
culture.
PBS without Ca and Mg, Sterile, 500 ml for cell culture.
MTT Proliferation Assay Kit, 1 Kutu 1000 Test. for cell culture
Cell culture flasks - surface area 25 cm2, Sterile,angled neck, cap (vented).
Cell culture flasks surface area 75 cm2, Sterile,canted neck, cap (plug seal).
96 well plate, Well Clear Microplate is comprised of clear TC-treated polystyrene. Crystal-grade
virgin polystyrene, Nonpyrogenic Flat bottom, with lid, individually wrapped, sterile, 50/case Tissueculture treated
Somatostatin-14 Sequence H-Ala-Gly-Cys-Lys-Asn-Phe-Phe-Trp-Lys-Thr-Phe-Thr-Ser-Cys-OH
acetate salt (Disulfide bond) CAS-Number 38916-34-6 (net), 25 mg
MAPK, kimyasal içeriği aşağıdaki gibi olmalı
Akt Rabbit Antibody Coated Microwells
Akt1 Mouse Detection Antibody
Anti-mouse IgG, HRP-linked Antibody
Phospho-Akt (S473) Rabbit Antibody Coated Microwells
Akt1 Mouse Detection Antibody
Anti-mouse IgG, HRP-linked Antibody
Akt Rabbit Antibody Coated Microwells
Phospho-Akt (T308) Mouse Detection Antibody
Anti-mouse IgG, HRP-linked Antibody
Phospho-p44/42 MAPK (T202/Y204) Rabbit Antibody Coated Microwells
p44/42 MAPK Mouse Detection Antibody
Anti-mouse IgG, HRP-linked Antibody
S6 Ribosomal Protein Mouse Antibody Coated Microwells
S6 Ribosomal Protein Rabbit Detection Antibody
Anti-rabbit IgG, HRP-linked Antibody
Phospho-S6 Ribosomal Protein (S235/236) Rabbit Antibody Coated Microwells
S6 Ribosomal Protein Mouse Detection Antibody
Anti-mouse IgG, HRP-linked Antibody
TMB Substrate 7004
STOP Solution 7002
Sealing Tape
ELISA Wash Buffer (20X)
ELISA Sample Diluent
Cell Lysis Buffer (10X) 9803
14.
16 tests
1.8 ml
1.8 ml
16 tests
1.8 ml
1.8 ml
16 tests
1.8 ml
1.8 ml
16 tests
1.8 ml
1.8 ml
16 tests
1.8 ml
1.8 ml
16 tests
1.8 ml
1.8 ml
11 ml
11 ml
2 sheets
25 ml
25 ml
15 ml
Green
Red
Green
Red
Green
Red
Green
Red
Green
Red
Green
Red
Colorless
Colorless
Colorless
Blue
Yellowish
EGFR, kimyasal içeriği aşağıdaki gibi olmalı
20X Wash Buffer Concentrate
300X HRP-Streptavidin Concentrate
5X Assay Diluent B
Assay Diluent A
Biotinylated anti-Human EGFR
EGFR Microplate (12 x 8 well strips)
Recombinant Human EGFR Standard
Stop Solution
TMB One-Step Substrate Reagent
1 x 25ml
1 x 200µl
1 x 15ml
1 x 30ml
2 vials
1 unit
2 vials
1 x 8ml
1 x 12ml
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10uL X-Long Vertex Tip; 10uL’lik gilson pipetman pipetlerine uygun tip. Certified RNase, DNase,
DNA and PCR inhibitor free, Certified non-pyrogenic, (1000tips/unit)
200uL Vertex Tip; Certified RNase, DNase, DNA and PCR inhibitor free, 100-200uL’lik pipetman
pipetlerine uygun tip, Certified non-pyrogenic (1000tips/unit)
1mL Vertex Tip, Certified RNase, DNase, DNA and PCR inhibitor free, 1000uL’lik pipetman
pipetlerine uygun tip, Certified non-pyrogenic (1000tips/unit)
10uL X-Long Filter Tip; 10uL’lik pipetman pipetlerine uçlarına uygun pipet kutusu; LOW
RETENTION; Certified RNase, DNase, DNA and PCR inhibitor free, Certified non-pyrogenic,
Sterilized, (96pcs/rack)
200uL Filter Tip; LOW RETENTION; 100-200uL’lik pipetman pipetlerine uçlarına uygun pipet
kutusu; Certified RNase, DNase, DNA and PCR inhibitor free, Certified non-pyrogenic, Sterilized,
(96pcs/rack)
1mL Filter Tip; 1000uL’lik pipetman pipetlerine uçlarına uygun pipet kutusu; LOW RETENTION;
Certified RNase, DNase, DNA and PCR inhibitor free, Certified non-pyrogenic, Sterilized,
(96pcs/rack)
Revolver 4-way rack Workstation;Add or subtract rack units to suit your needs.PCR plate
configuration. Rotate and lock mechanism. Fast Reconfiguration for different tube sizes and types.
Material: Polypropylene. Temp range -80°C to +121°C.Autoclavable.
Cell lifter, Used for scraping cells from dishes and plates. Ideal tool for harvesting stem cells and
colonies while maintaining high viability.One piece design for better control. Blade design minimizes
cell damage and ensures even contact with the growth surface. Individually wrapped. Sterilized by
gamma radiation and certified nonpyrogenic
Petri Dish, Sterile,100 mm, 420/cs. Approximate growth surface area is 55cm², Actual inside growth
surface diameter is 80.5mm, Height is 20mm, Manufactured from optically-clear virgin polystyrene,
Treated for optimal cell attachment, Sterilized by gamma radiation and certified nonpyrogenic, Have
stacking beads to aid in handling, Supplied with vents to provide consistent gas exchange
15 ml'lik santrifüj
tüpü,Doublethreadcapreducescrossthreading.Opticallycleartoimprovespecimenvisibility.Largerwritin
gpatchforsample ID.Polypropylenetubeswithstandcentrifugalforces, 16,000 RCF.Sampleintegrity is
maintained in extremelylowtemperatureto -80ºC.
50 ml'lik santrifüj
tüpü,Doublethreadcapreducescrossthreading.Opticallycleartoimprovespecimenvisibility.Largerwritin
gpatchforsample ID.Polypropylenetubeswithstandcentrifugalforces, 16,000 RCF.Sampleintegrity is
maintained in extremelylowtemperatureto -80ºC.
Serological pipets–individually wrapped*5 ml (1 kutu 200 adet), Sterile,suitable for cell culture.
Serological pipets–individually wrapped*10ml (1 kutu 200 adet), Sterile,suitable for cell culture.
Serological pipets–individually wrapped*25 ml (1 kutu 200 adet), Sterile,suitable for cell culture.
Thiazolyl Blue Tetrazolium Bromide, 500mg, 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2Htetrazolium bromide, MTT, Methylthiazolyldiphenyl-tetrazolium bromide, powder, BioReagent,
suitable for cell culture, suitable for insect cell culture, ≥97.5% (HPLC),
5 ml’lik Ependorf, 5 mL Microcentrifuge Tube, Assorted (Red, Blue, Green,Yellow),4 bags of 50
tubes/unit
31.
Gambogic acid 5 mg, ≥95% (HPLC), powderSynonym: 1,5-Methano-1H,3H,11H-furo(3,4g)pyrano(3,2-b)xanthene-1-crotonic acid, B"-Guttiferrin, Beta-Guttiferrin, Beta-Guttilactone,
Cambogic acid, Guttatic acid, Guttic acid